Cellufine™ hydrophobic interaction chromatography (HIC) media are porous chromatography particles, comprising cross-linked cellulose covalently bonded to either a butyl or phenyl functionality via a short spacer.
Factors which affect the hydrophobic interactions of the media include salt concentration, temperature, pH and the presence of surfactants and organic solvents. Typically, the higher the ionic strength (salt concentration), the stronger the hydrophobic bonding. Consequently, the interactions are enhanced by conditions that are the inverse of those for ion exchange chromatography. HIC is, therefore, an effective and complementary tool for separating and purifying substances which are difficult to or impossible to separate by ion exchange chromatography.
Cellufine™ MAX HIC Phenyl media is a next-generation Cellufine™ media with high flow characteristics. Cellufine™ MAX HIC Phenyl media with two types of functional ligand density is available: standard ligand density and low ligand density (LS).