产品&服务 BioOptimal™ MF-SL 微型过滤器
使简单有效的净化成为可能
BioOptimal MF-SL 是一款中空纤维过滤器，采用孔径大小筛选和切向流过滤的高效过滤机制。这两个方面使得过滤器能够平稳有效地分离溶液成分。
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应用
工艺简化
BioOptimal MF-SL 能够在单个工艺步骤中快速完整地澄清溶液。因此可以简化细胞培养净化工艺，并通过排除离心机等大型投资和深度过滤器等昂贵的一次性物品的需求，使其更具成本效益。
特点&性能
主要特点
- 低TMP（跨膜压力）
- 流量高，过滤时间短
- 高蛋白质渗透率 & 回收率
- 过滤液浑浊度非常低，微生物较少
- 过滤工艺中的低压应力
基本信息
切向流过滤
进料流体流过膜表面产生清扫作用，防止快速堵塞并延长过滤时间。
BioOptimal MF-SL 结构和切向流过滤的说明。
性能
清除
Filtration Volume vs. Filtration Time
Test Conditions:
Cell Culture Type : CHO-S
Filtration Mode : Tangential Flow Filtration
Shear Rate : 3000 s-1
TMP : No Control (= 3-6 KPa)
Cell Culture Type : CHO-S
Filtration Mode : Tangential Flow Filtration
Shear Rate : 3000 s-1
TMP : No Control (= 3-6 KPa)
| Cell Density x 106 (cells/mL) | Cell Viability (%) | Turbidity (NTU) | ||
|---|---|---|---|---|
| Before | After | |||
| Low viability | 13.9 | 26 | 1578 | 16.7 |
| Medium viability | 18.5 | 87.2 | 1806 | 5.2 |
| High viability | 11 | 99.5 | 1010 | 4.3 |
TFF灌注细胞培养评估
单克隆抗体（mAb）是生物制药领域的主要研究对象之一。有几种类型的细胞培养系统，如补料分批和灌流培养。虽然传统的批次系统在当前的培养工艺中被广泛接受，但基于灌流细胞培养系统为长期大大增加细胞密度和生产力提供了潜力。中空纤维在灌流培养中作为细胞分离装置被广泛接受。我们对在TFF（切向流过滤）系统中灌流细胞培养的性能进行了评估。TFF Perfusion set-up
Test Conditions:
Cell culture: Antibody-producing Chinese hamster ovary (CHO) cells were cultivated in serum free media.
Cell density was maintained at 50-70 ×106 cells/mL by bleeding.
Medium exchange rate: 0.75-2.5 vessel/day
Permeate flux: 0.24-0.80 L/m2/h (LMH)
Bioreactor working volume was adjusted by membrane area.
Flow rate (L/min) was adjusted so that the shear rate inside of hollow fiber became 1500 s-1.
Cell culture: Antibody-producing Chinese hamster ovary (CHO) cells were cultivated in serum free media.
Cell density was maintained at 50-70 ×106 cells/mL by bleeding.
Medium exchange rate: 0.75-2.5 vessel/day
Permeate flux: 0.24-0.80 L/m2/h (LMH)
Bioreactor working volume was adjusted by membrane area.
Flow rate (L/min) was adjusted so that the shear rate inside of hollow fiber became 1500 s-1.
Figure mAb Permebility
规格&参数
材料规格
| Pore size (μm) | 0.4 |
|---|---|
| Hollow fiber ID (mm) | 1.4 |
| Hollow fiber material | Polysulfone |
| Hollow fiber housing | Polysulfone |
订购信息
产品型号
| Catalog Number | Effective Membrane Surface Area Size |
|---|---|
| MFSL0005 | 0.005 m² |
| MFSL0190 | 0.19 m² |
| MFSL1000 | 1 m² |
| MFSL8000 | 8 m² |
