Seamless Scale-Up of Hollow Fiber Microfilters for N Perfusion Cell Culture in TFF Mode

This application note provides key considerations for scaling TFF-based N perfusion processes using hollow fiber microfilters. It explains how pressure drop is affected by critical design factors—including flow rate, tubing length and diameter, fluid viscosity, and microfilter dimensions—and how these factors should be managed to maintain a stable hydraulic environment across scales. A practical four-stage workflow is also introduced to guide the transition from development scale to pilot scale, supporting consistent performance and robust scale-up.