News
Analyzing the filtration behavior of different-sized aggregates using Planova™ virus removal filters
Jun 03, 2024
Jun 3, 2024
Asahi Kasei Medical Co., Ltd.
Asahi Kasei Medical Co., Ltd. (Headquarters: Chiyoda-ku, Tokyo; President: Ken Shinomiya) is pleased to announce the publication of a study that analyzed the filtration behavior of Planova™ virus removal filters using different-sized aggregates during filtration.
The effect of protein aggregates on the filtration performance of virus removal filters is widely known. However, there are few studies on how different-sized aggregates are retained in the filter and how this retention affects filtration performance.
Tsukamoto and colleagues used size exclusion chromatography to fractionate aggregates into different sizes, and then filtered through a Planova 20N filter. The aggregates were analyzed for their effect on filtration behavior and where they were retained on the filter membrane. From these results, new observations were obtained on the blocking mechanism of virus removal filters that have a gradient structure with pore diameter gradually decreasing in the direction of the membrane thickness.
Through our findings, we can further elucidate membrane fouling and virus removal mechanisms using the analytical approaches introduced in this study. At Asahi Kasei Medical, we plan to continue our efforts and endeavors into research and development, creating high quality and functional products to ensure the safety of biotherapeutics worldwide.
*This study is published in Biotechnology Progress.
Title: Size-based analysis of virus removal filter fouling using fractionated protein aggregates
(Open Access: https://doi.org/10.1002/btpr.3391)
The effect of protein aggregates on the filtration performance of virus removal filters is widely known. However, there are few studies on how different-sized aggregates are retained in the filter and how this retention affects filtration performance.
Tsukamoto and colleagues used size exclusion chromatography to fractionate aggregates into different sizes, and then filtered through a Planova 20N filter. The aggregates were analyzed for their effect on filtration behavior and where they were retained on the filter membrane. From these results, new observations were obtained on the blocking mechanism of virus removal filters that have a gradient structure with pore diameter gradually decreasing in the direction of the membrane thickness.
Through our findings, we can further elucidate membrane fouling and virus removal mechanisms using the analytical approaches introduced in this study. At Asahi Kasei Medical, we plan to continue our efforts and endeavors into research and development, creating high quality and functional products to ensure the safety of biotherapeutics worldwide.
*This study is published in Biotechnology Progress.
Title: Size-based analysis of virus removal filter fouling using fractionated protein aggregates
(Open Access: https://doi.org/10.1002/btpr.3391)
