BioOptimal™ UMP / MF-SL / UJP Microfilters
Process Intensification Using BioOptimal Microfilters
In Perfusion Cell Culture
BioOptimal™ microfilters are ideal for N perfusion in intensified upstream processing strategies, including Tangential Flow Filtration (TFF), reverse Tangential Flow Filtration (rTFF), and Alternating Tangential Flow (ATF) clarification modes. Additionally, BioOptimal microfilters are compatible with other common upstream culture strategies in use today, including N-1 perfusion, fed-batch and traditional batch processes.
- High yield - Low membrane clogging × High product sieving
- High permeate quality - Low turbidity in permeate
- Low damage or shear to cells - High viable cell density and viability
Product Lineup
FOR N PERFUSION
Optimized for TFF and rTFF in N perfusion, delivering high sieving and long, stable filtration in high density cell cultures.
Optimized for TFF and rTFF in N perfusion, delivering high sieving and long, stable filtration in high density cell cultures.
FOR N PERFUSION
Flexibly designed for TFF, rTFF and ATF in perfusion culture, offering both high sieving and long, stable filtration.
Flexibly designed for TFF, rTFF and ATF in perfusion culture, offering both high sieving and long, stable filtration.
FOR N-1 PERFUSION
Ideal for N-1 perfusion applications, providing long and stable filtration even under high flux conditions.
Ideal for N-1 perfusion applications, providing long and stable filtration even under high flux conditions.
| BioOptimal UMP | BioOptimal MF-SL | BioOptimal UJP | |
|---|---|---|---|
| Hollow fiber material | Polyvinylidene fluoride (PVDF) |
Polysulfone (PS) |
Polyvinylidene fluoride (PVDF) |
| Nominal pore size (μm) | 0.2 | 0.4 | 0.65 |
| Inner diameter (mm) | 1.4 | 1.4 | 1.1 |
| Effective surface area (m2) | 8.8, 3.8, 1.9, 0.77, 0.34, 0.19, 0.09, 0.02 |
8, 1, 0.19, 0.005 | 3.7, 1.8, 0.76, 0.32, 0.19, 0.1, 0.02 |
| Max operating pressure | 300 kPa, 43.5 psi | 200 kPa, 29.0 psi | 300 kPa, 43.5 psi |
| Cleaning/sterilization procedures | CIP, SIP (125℃) | CIP, SIP (123℃) | CIP, SIP (125℃) |
PVDF : Polyvinylidene fluoride, PS : Polysulfone
Features
BioOptimal UMP | High Product Sieving in N Perfusion
BioOptimal UMP is a high-performance hollow fiber microfilter specifically designed for TFF and rTFF in perfusion culture processes. Engineered for process intensification, it offers exceptional antibody permeability (product sieving), enabling both cost reduction and increased product yield.
・Monoclonal antibody N perfusion case study
BioOptimal UMP maintained >90% product sieving during 32 days of perfusion culture.
・Monoclonal antibody N perfusion case study: customer data [1]
BioOptimal UMP shows antibody sieving coefficient >85% throughout 40 days, independent of the crossflow shear rates and permeate fluxes studied.Figure adapted to show only BioOptimal UMP data.
[1] BioOptimal UMP is shown as Asahi Kasei Microza 0.2μm UMP in the original paper. Pinto NDS, Brower M. Wide-surface pore microfiltration membrane drastically improves sieving decay in TFF-based perfusion cell culture and streamline chromatography integration for continuous bioprocessing. Biotechnology and Bioengineering. 2020; 117: 3336-3344.
BioOptimal MF-SL
BioOptimal MF-SL is our original hollow fiber microfilter that also achieves high product sieving in N perfusion cultures, enabling improved manufacturing efficiency by way of process intensification. A versatile microfilter, BioOptimal MF-SL demonstrates excellent performance in TFF, rTFF and ATF applications, thereby supporting most intensification methods today.
・Monoclonal antibody N perfusion internal case study: TFF mode
BioOptimal MF-SL maintained product sieving above 80% for >27 days of perfusion culture in TFF mode.
Documents
Technical Documents
Access technical documents for BioOptimal microfilters through the links below.
Related Presentations
Related Publications
Access publications on BioOptimal microfilters through the links below.
- Wide-surface pore microfiltration membrane drastically improves sieving decay in TFF-based perfusion cell culture and streamline chromatography integration for continuous bioprocessing
- Effect of inner diameter, filter length, and pore size on hollow fiber filter fouling during perfusion cell culture
Brochure
Download brochures on BioOptimal microfilters.
Product Specifications
BioOptimal UMP
| Effective surface area (m2) | 8.8 | 3.8 | 1.9 | 0.77 | 0.34 | 0.19 | 0.09 | 0.02 | |
|---|---|---|---|---|---|---|---|---|---|
| Component | Hollow fiber membrane | Polyvinylidene fluoride (PVDF) (Inner diameter: 1.4 ± 0.1 mm, nominal pore size: 0.2 μm) |
|||||||
| Hollow fiber sleeves | Polysulfone | --a | |||||||
| Hollow fiber net | -- | Perfluoroalkoxy alkane (PFA) | -- | ||||||
| Housing, housing ends and headers | Polysulfone | ||||||||
| Lock nuts | Polyphenylene ether and glass fiber | -- | |||||||
| Sealant | Epoxy resin | ||||||||
| O-rings | Ethylene propylene diene monomer (EPDM) | ||||||||
| Ferrule caps (feed and retentate sides) |
Polypropylene | Polysulfone | Polypropylene | ||||||
| Ferrule caps (permeate side) |
Polysulfone | Stainless steel | Polypropylene | --a | |||||
| Nozzle caps (permeate side) |
-- | Silicone | |||||||
| Ferrule gaskets | Ethylene propylene diene monomer (EPDM) | ||||||||
| Clamp bands | Stainless steel | Polyamide and glass fiber | |||||||
| Supplied as | Hollow fiber membrane wetted with 17% (w/w) ethanol | Filled with 17% (w/w) ethanol | |||||||
| Quantity of 17% (w/w) ethanol (L) | 4.3 | 1.8 | 0.9 | 0.4 | 0.5 | 0.5 | 0.3 | 0.03 | |
| Sterilization method | Not sterilized | ||||||||
| Packaging format | Packed individually in bags | ||||||||
a Not applicable
BioOptimal MF-SL
| Effective surface area (m2) | 8 | 1 | 0.19 | 0.005 | ||
|---|---|---|---|---|---|---|
| Component | Hollow fiber membrane | Polysulfone | ||||
| Housing and header | --a | Polysulfone | ||||
| Housing end, housing body, header, hollow fiber sleeve | Polysulfone | -- | ||||
| Sealant | Epoxy resin | |||||
| O-rings | Ethylene propylene diene monomer (EPDM) | Silicone | -- | |||
| Lock nuts | Polyphenylene ether and glass fiber | Polysulfone | -- | |||
| Ferrule caps | Polysulfone (2S) Polypropylene (3S) |
Polycarbonate | -- | |||
| Ferrule gaskets | Ethylene propylene diene monomer (EPDM) | -- | ||||
| Nozzle caps | -- | Polypropylene | ||||
| Luer lock plugs | -- | Polypropylene | ||||
| Nozzle stoppers (feed side) | -- | Silicone | -- | |||
| Nozzle stoppers (permeate side) | -- | Silicone | -- | |||
| Clamp bands | Stainless steel | Polysulfone, glass fiber | -- | |||
| Threaded clamps | Clamp bolts | -- | -- | Polypropylene | -- | |
| Clamp nuts | -- | -- | Polypropylene | -- | ||
| Microfilter state | Wetted with 17% (w/w) ethanol | |||||
| Quantity of 17% (w/w) ethanol (mL) | 4000 | 530 | 100 | 2.3 | ||
| Packing format | Packed individually in boxes | |||||
a Not applicable
BioOptimal UJP
| Effective surface area (m2) | 3.7 | 1.8 | 0.76 | 0.32 | 0.19 | 0.1 | 0.02 | |
|---|---|---|---|---|---|---|---|---|
| Component | Hollow fiber membrane | Polyvinylidene fluoride (PVDF) (Inner diameter: 1.1 ± 0.1 mm, nominal pore size: 0.65 μm) |
||||||
| Hollow fiber sleeves | Polysulfone | --a | ||||||
| Hollow fiber net | Perfluoroalkoxy alkane (PFA) | -- | ||||||
| Housing, housing ends and headers | Polysulfone | |||||||
| Sealant | Epoxy resin | |||||||
| O-rings | Ethylene propylene diene monomer (EPDM) | |||||||
| Ferrule caps (feed and retentate sides) |
Polysulfone | Polypropylene | ||||||
| Ferrule caps (permeate side) |
Polysulfone | Stainless steel | Polypropylene | -- a | ||||
| Nozzle caps (permeate side) |
-- | Silicone | ||||||
| Ferrule gaskets | Ethylene propylene diene monomer (EPDM) | |||||||
| Clamp bands | Stainless steel | Polyamide and glass fiber | ||||||
| Supplied as | Hollow fiber membrane wetted with 17% (w/w) ethanol | Filled with 17% (w/w) ethanol | ||||||
| Quantity of 17% (w/w) ethanol (L) | 1.8 | 0.9 | 0.4 | 0.5 | 0.5 | 0.3 | 0.03 | |
| Sterilization method | Not sterilized | |||||||
| Packaging format | Packed individually in bags | |||||||
a Not applicable
Related Products & Services
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Ordering Information
Please reference the catalog number when placing orders.
| Filter type | Effective surface area (m²) | Catalog number |
|---|---|---|
| BioOptimal UMP | 8.8 | UMP5247R |
| 3.8 | UMP3247R | |
| 1.9 | UMP3147R | |
| 0.77 | UMP2147R | |
| 0.34 | UMP1147M | |
| 0.19 | UMP1147R | |
| 0.09 | UMP1047R | |
| 0.02 | UMP0047R | |
| BioOptimal MF-SL | 8 | MFSL8000 |
| 1 | MFSL1000 | |
| 0.19 | MFSL0190 | |
| 0.005 | MFSL0005 | |
| BioOptimal UJP | 3.7 | UJP3247R |
| 1.8 | UJP3147R | |
| 0.76 | UJP2147R | |
| 0.32 | UJP1147M | |
| 0.19 | UJP1147R | |
| 0.1 | UJP1047R | |
| 0.02 | UJP0047R |
